Forensic Science International - Articles in Press

Quantitative analysis of methamphetamine in hair of children removed from clandestine laboratories – Evidence of passive exposure? - Corrected Proof

T. Bassindale — 2012-02-03

Abstract: In New Zealand many children have been removed from clandestine laboratories following Police intervention. In the last few years it has become standard procedure that these children have hair samples taken and these samples are submitted to the laboratory for analysis.There are various mechanisms for the incorporation of drugs into hair. The hair follicle has a rich blood supply, so any drug that may be circulating in the blood can be incorporated into the growing hair. Another mechanism is via external contamination, such as spilling a drug on the hair or through exposure to fumes or vapours.Hair samples were analysed for methamphetamine and amphetamine. From the 52 cases analysed 38 (73%) were positive for methamphetamine (>0.1ng/mg) and amphetamine was detected in 34 of these cases. In no case was amphetamine detected without methamphetamine. The hair washes (prior to extraction) were also analysed (quantified in 30 of the positive cases) and only 3 had a wash to hair ratio of >0.1 (all were <0.5), which may be indicative of a low level of external contamination. This low level of evidence of external contamination suggests that the children are exposed to methamphetamine and are incorporating it into the hair through the blood stream.

The first international proficiency test on ketamine and norketamine in hair - Corrected Proof

2012-02-03

Abstract: This paper discusses the organization of the first international proficiency test (PT) programme on ketamine (K) and norketamine (NK) in hair samples. The primary objective of the programme was to evaluate the analytical capability of participating laboratories on hair analysis for K and NK via comparison of results. Authentic samples, instead of spiked samples were used in the programme to mimic the analysis of incorporated illicit drugs in real-life situations. Eight of the ten participating medical or forensic laboratories from Australia, France, Hong Kong, Italy, Singapore and the USA returned results to the organizer. Quantification methods from these laboratories were confined to GC–MS and LC–MS/MS. Performance assessment based on z-score indicated that only three laboratories achieved satisfactory results for both the analysis of K and NK. It was concluded that the overall performance of the participating laboratories was fair and there is still room for further improvement. Additional similarly designed PT programmes are recommended to be organized in order to encourage reliable measurements of illicit drugs in hair samples. Taking into account the substantial effect on the consensus values within limited number of data points, a recommendation on the provision of reference values assigned by accurate methods will be of benefit to small size PT programmes in the forensic field.

Morphology of preimaginal stages of Calliphora vicina Robineau-Desvoidy, 1830 (Diptera, Calliphoridae): A comparative study - Corrected Proof

2012-02-02

Abstract: A comparative morphological study of preimaginal stages (larvae and pupae) of Calliphora vicina Robineau-Desvoidy, 1830 is presented. The entomological samples came from laboratory colonies bred under controlled environmental conditions (25°C and 60% relative humidity). In this study, a recently published technique to clear Diptera larvae for light microscopy and a standard protocol for scanning electron microscopy were used. For the morphological comparison of larval instars I, II and III, and pupae of C. vicina, different larval regions (cephalic, thoracic and abdominal, including anal division), as well the internal chitinised cephalopharyngeal skeleton, were considered separately. Our results focus on showing the changes observed throughout development for the most important structures in the cephalic region (sensilla of maxillary palpus, antennae and oral ridges), the thoracic region (the first segment and its anterior spinose band) and in the anal division of the abdominal region (posterior spiracles and shape of the papillae). In addition, some morphological structures are described or pictured for the first time, such as the ventral organ and the anterior spiracle of larva I and the antenna sensilla, Keilin's organ and wrinkled area of the anal division of all instars. The cephalopharyngeal skeleton is an important structure for the taxonomy of Diptera larvae in all instars, including Calliphoridae. Our observations in C. vicina indicate that an in-depth review of the sclerite composition is needed. Pupae and larvae stages can only be compared by following the segmentary spinose bands and the anal segment, where the morphology of the posterior spiracles and papillae can be observed, in some cases despite the reduced condition of the latter.

Score-based likelihood ratios for handwriting evidence - Corrected Proof

Amanda B. Hepler, Christopher P. Saunders, Linda J. Davis, JoAnn Buscaglia — 2012-02-01

Abstract: Score-based approaches for computing forensic likelihood ratios are becoming more prevalent in the forensic literature. When two items of evidential value are entangled via a scorefunction, several nuances arise when attempting to model the score behavior under the competing source-level propositions. Specific assumptions must be made in order to appropriately model the numerator and denominator probability distributions. This process is fairly straightforward for the numerator of the score-based likelihood ratio, entailing the generation of a database of scores obtained by pairing items of evidence from the same source. However, this process presents ambiguities for the denominator database generation – in particular, how best to generate a database of scores between two items of different sources.Many alternatives have appeared in the literature, three of which we will consider in detail. They differ in their approach to generating denominator databases, by pairing (1) the item of known source with randomly selected items from a relevant database; (2) the item of unknown source with randomly generated items from a relevant database; or (3) two randomly generated items. When the two items differ in type, perhaps one having higher information content, these three alternatives can produce very different denominator databases. While each of these alternatives has appeared in the literature, the decision of how to generate the denominator database is often made without calling attention to the subjective nature of this process.In this paper, we compare each of the three methods (and the resulting score-based likelihood ratios), which can be thought of as three distinct interpretations of the denominator proposition. Our goal in performing these comparisons is to illustrate the effect that subtle modifications of these propositions can have on inferences drawn from the evidence evaluation procedure. The study was performed using a data set composed of cursive writing samples from over 400 writers. We found that, when provided with the same two items of evidence, the three methods often would lead to differing conclusions (with rates of disagreement ranging from 0.005 to 0.48). Rates of misleading evidence and Tippet plots are both used to characterize the range of behavior for the methods over varying sized questioned documents. The appendix shows that the three score-based likelihood ratios are theoretically very different not only from each other, but also from the likelihood ratio, and as a consequence each display drastically different behavior.

Three-dimensional computer-assisted craniometrics: A comparison of the uncertainty in measurement induced by surface reconstruction performed by two computer programs - Corrected Proof

2012-02-01

Abstract: Digital investigation of anthropological material through computed tomography (CT) offers several new opportunities in morphometrics. However, an object measured with computer-assisted methods does not necessarily exactly match the original one. The scanning and surface reconstruction of the object induce some alterations, and data acquisition is prone to measurement uncertainty. The purpose of this research is to evaluate the intra- and inter-observers variations in medical CT scan measurements of a known-size phantom and two dry crania. Two software packages, AMIRA and Treatment and Increased Vision for Medical Imaging (TIVMI), which use different techniques of surface reconstructions, were compared. The mean difference between the measurements was lower for TIVMI, using an objective algorithm based on the half-maximum height (HMH) protocol in three dimensions (3D). AMIRA can induce up to a 4% error in known measurements and 5% uncertainty in dry skull measurements. This study emphasises the risk of object shape alteration in each step of its digitisation.

Detection of drugs of abuse in simultaneously collected oral fluid, urine and blood from Norwegian drug drivers - Corrected Proof

2012-01-30

Abstract: Blood and urine samples are collected when the Norwegian police apprehend a person suspected of driving under the influence of drugs other than alcohol. Impairment is judged from the findings in blood. In our routine samples, urine is analysed if morphine is detected in blood to differentiate between ingestion of heroin, morphine or codeine and also in cases where the amount of blood is too low to perform both screening and quantification analysis. In several cases, the collection of urine might be time consuming and challenging. The aim of this study was to investigate if drugs detected in blood were found in oral fluid and if interpretation of opiate findings in oral fluid is as conclusive as in urine.Blood, urine and oral fluid samples were collected from 100 drivers suspected of drugged driving. Oral fluid and blood were screened using LC–MS/MS methods and urine by immunological methods. Positive findings in blood and urine were confirmed with chromatographic methods. The analytical method for oral fluid included 25 of the most commonly abused drugs in Norway and some metabolites.The analysis showed a good correlation between the findings in urine and oral fluid for amphetamines, cocaine/benzoylecgonine, methadone, opiates, zopiclone and benzodiazepines including the 7-amino-benzodiazepines. Cocaine and the heroin marker 6-monoacetylmorphine (6-MAM) were more frequently detected in oral fluid than in urine. Drug concentrations above the cut-off values were found in both samples of oral fluid and urine in 15 of 22 cases positive for morphine, in 18 of 20 cases positive for codeine and in 19 of 26 cases positive for 6-MAM. The use of cannabis was confirmed by detecting THC in oral fluid and THC-COOH in urine. In 34 of 46 cases the use of cannabis was confirmed both in oral fluid and urine. The use of cannabis was confirmed by a positive finding in only urine in 11 cases and in only oral fluid in one case. All the drug groups detected in blood were also found in oral fluid.Since all relevant drugs detected in blood were possible to find in oral fluid and the interpretation of the opiate findings in oral fluid was more conclusive than in urine, oral fluid might replace urine in driving under the influence cases. The fast and easy sampling is time saving and less intrusive for the drivers.

Styryl dye coated metal oxide powders for the detection of latent fingermarks on non-porous surfaces - Corrected Proof

2012-01-30

Abstract: Conventional fingermark powders rely on contrast induced by absorption/reflection (e.g. black powder) or luminescence in the visible region (e.g. Blitz Green®). In most cases, these powders provide sufficient contrast; however, in some circumstances surface characteristics can interfere with the visualisation of powdered fingermarks. Visualisation in the near infra-red (NIR) region, however, has been shown to eliminate interferences commonly encountered in the visible region. In this study, a mixture of rhodamine 6G and the NIR laser dye styryl 11 (STaR 11) was coated onto an aluminium oxide nanopowder and then mixed with silver magnetic powder to develop and visualise fingermarks in the NIR. When compared to Blitz Green®, it was determined that the STaR 11 magnetic powder was better suited for marks deposited on textured surfaces and for older marks, whereas Blitz Green® performed better on smooth glossy surfaces. The ability of the STaR 11 mixed dye formulation to be visualised in both the visible and NIR regions also provides a significant advantage over conventional luminescent fingermark powders.

Establishing forensic search methodologies and geophysical surveying for the detection of clandestine graves in coastal beach environments - Corrected Proof

Jamie K. Pringle, Claire Holland, Katie Szkornik, Mark Harrison — 2012-01-30

Abstract: A 2010 UK police search for a clandestine burial highlighted the need for more information and quantitative data to aid coastal beach searches. This study aimed to address this by establishing relevant forensic search methodologies to aid the search for clandestine coastal burial sites, using the North West English coastline as a search area. A set of parameters were established, including criteria such as tidal range, proximity to vehicular access points and distance from inhabited areas, which may inform forensic searches by prioritising likely locations of clandestine burials. Three prioritised coastal locations were subsequently identified: (1) coastal dunes at Formby, (2) coastal dunes and (3) beach foreshore at Southport, all sites part of the Liverpool City Region in the United Kingdom. At all locations, simulated clandestine graves were hand-dug by spades into which a naked adult-sized, metal-jointed fiberglass mannequin was buried at 0.5m below ground level. Trial geophysical surveys were then undertaken with the aim of identifying the optimal geophysical instrumentation and technique to deploy in such environments. GPR data showed 450MHz frequency antennae to be optimal, with significantly poor data obtained from the foreshore area due to saline seawater. Electrical resistivity and magnetic susceptibility surveys were successful in coastal environments in target detection (albeit not in non-vegetated sand dunes), with resistivity fixed-offset configurations deemed optimal. The latter survey successes may be due to the recent disturbed ‘grave’ rather than the target, which itself is of interest in terms of identifying the most recent clandestine burials.

Factitious disorder comorbid with bipolar I disorder. A case report - Corrected Proof

2012-01-30

Abstract: We describe a case of factitious disorder with physical and psychological symptoms comorbid with bipolar I disorder in a 37-year-old woman. Since the onset of bipolar disorder, which occurred at the age of 31, she increasingly complained of physical symptoms, compulsively seeking medical and surgical interventions. She has been hospitalised several times and her Munchausen-type factitious disorder recently appeared to be developing into Munchausen by proxy, involving her 11-year-old daughter. The patient adhered poorly to stabilising and antipsychotic drug treatment and did not improve through the years. We here analyse her mood phases, which were always associated with changes in the quality of factitious symptoms, according to whether the disorder was in its depressive phase (somatic complaints and suicidal ideation prevail), or in its manic or mixed phase (medical intervention-seeking and manipulation of clinicians to obtain surgical interventions). We also briefly discuss some important forensic issues to consider in similar cases, mainly stemming from the psychotic aspects of these two co-occurring disorders. Clinicians should be aware of some patients’ ability to produce signs and symptoms of physical and/or psychological illness and consult psychiatrists before giving consent to invasive diagnostic procedures or surgery.

Evaluation of human brain damage in fatalities due to extreme environmental temperature by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP), S100β and single-stranded DNA (ssDNA) immunoreactivities - Corrected Proof

Qi Wang, Takaki Ishikawa, Tomomi Michiue, Bao-Li Zhu, Da-Wei Guan, Hitoshi Maeda — 2012-01-30

Abstract: Fatalities due to extreme environmental temperatures involving hypothermia (cold exposure) and hyperthermia (heat stroke) might present with poor or nonspecific morphological pathologies, which are insufficient to establish the cause of death in forensic practice. The present study immunohistochemically investigated basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP), S100β and single-stranded DNA (ssDNA) in the parietal lobe and hippocampus of the brain in fatalities from hypothermia (n=15) and hyperthermia (n=18), and compared them to those of controls (n=39), including acute death due to ischemic heart disease, mechanical asphyxiation and drowning. In addition, S100β concentration in cerebrospinal fluid (CSF) was measured. Characteristic findings in hypothermia cases were higher glial bFGF immunopositivity in the cerebral cortex and white matter, and higher S100β immunopositivity in the cerebral cortex with a lower CSF S100β concentration. Hyperthermia showed lower glial GFAP and S100β immunopositivities in the white matter, and higher neuronal ssDNA immunopositivity in the cerebral cortex and hippocampus, accompanied by high glial bFGF and S100β immunopositivities in the cerebral cortex. These findings suggest neuroprotective glial responses without marked neuronal or glial damage in fatal hypothermia, and diffuse neuronal apoptosis despite initiation of neuroprotective cortical astrocyte responses, accompanied by glial damage in the white matter, in fatal hyperthermia. These markers may be useful for evaluating brain damage and responses in fatalities due to extreme environmental temperatures.

Evaluation of postmortem redistribution phenomena for commonly encountered drugs - Corrected Proof

2012-01-30

Abstract: We described the findings of a study into the post-mortem redistribution (PMR) of 76 drugs found in 129 drug-related cases between 2006 and 2009. Seventy six drugs (psychotropic drugs (n=14), antidepressants (n=9), sedatives (n=6) and so on) were simultaneously quantified in cardiac and peripheral blood by gas chromatography–mass spectrometry (GC/MS) or liquid chromatography–tandem mass spectrometry (LC/MS/MS). The absence, possibility or presence of PMR of drugs was determined according to the ratios of cardiac to femoral blood concentrations (C/P ratios). Proxyphylline (C/P ratio: 0.85) showed no PMR; carbamazepine was not subject to PMR; a potential for PMR of lorazepam and mirtrazapine cannot be excluded; chlordiazepoxide is subject to PMR; acetaminophen and alprazolam exhibit minimal PMR; amitriptyline and benztropine exhibit PMR. Codeine (C/P ratio: 4.9), zolpidem (C/P ratio: 3.74), chlorpromazine (C/P ratio: 2.97), fluoxetine (C/P ratio: 2.83) and propranolol (C/P ratio: 2.72) had the largest C/P ratios.Postmortem drug concentrations showed variations depending on sampling sites and characteristics of the drugs. It is continuously necessary to analyze commonly used or abused drugs in simultaneously collected cardiac and peripheral blood to establish significant reference values for PMR. These findings can be used to reach a conclusion about the cause and manner of death.

Investigation of morphine and morphine glucuronide levels and cytochrome P450 isoenzyme 2D6 genotype in codeine-related deaths - Corrected Proof

Joachim Frost, Arne Helland, Ivar S. Nordrum, Lars Slørdal — 2012-01-30

Abstract: Compared to morphine and morphine-6-glucuronide (M6G), codeine and its other major metabolites codeine-6-glucuronide and norcodeine have weak affinity to opioid μ-receptors. Analgesic effects of codeine are thus largely dependent on metabolic conversion to morphine by the polymorphic cytochrome P450 isoenzyme 2D6 (CYP2D6). How this relates to toxicity and post-mortem whole blood levels is not known. This paper presents a case series of codeine-related deaths where concentrations of morphine, M6G and morphine-3-glucuronide (M3G), as well as CYP2D6 genotype, are taken into account. Post-mortem toxicological specimens from a total of 1444 consecutive forensic autopsy cases in Central Norway were analyzed. Among these, 111 cases with detectable amounts of codeine in femoral blood were identified, of which 34 had femoral blood concentrations exceeding the TIAFT toxicity threshold of 0.3mg/L. Autopsy records of these 34 cases were retrieved and reviewed. In the 34 reviewed cases, there was a large variability in individual morphine to codeine concentration ratios (M/C ratios), and morphine levels could not be predicted from codeine concentrations, even when CYP2D6 genotype was known. 13 cases had codeine concentrations exceeding the TIAFT threshold for possibly lethal serum concentrations (1.6mg/L). Among these, 8 individuals had morphine concentrations below the toxic threshold according to TIAFT (0.15mg/L). In one case, morphine as well as M6G and M3G concentrations were below the limit of detection. A comprehensive investigation of codeine-related fatalities should, in addition to a detailed case history, include quantification of morphine and morphine metabolites. CYP2D6 genotyping may be of interest in cases with unexpectedly high or low M/C ratios.

Quantitative monitoring of corticosteroids in cosmetic products manufactured in Korea using LC–MS/MS - Corrected Proof

Yun Sik Nam, Il Keun Kwon, Yeonhee Lee, Kang-Bong Lee — 2012-01-27

Abstract: Some cosmetic products manufactured in Korea for the treatment of eczema, seborrhea and psoriasis have been suspected to contain anti-inflammatory corticosteroids such as prednisolone, hydrocortisone, betamethasone, dexamethasone and triamcinolone acetonide without these ingredients being indicated on the label. Due to their severe side effects such as permenent skin atopy, these corticosteroids have to be monitored in cosmetic products from a forensic point of view. Many cosmetic product samples (N=65) have been collected from both local and online markets in Korea. The corticosteroid content of these samples was analyzed by LC–MS/MS with diagnostic ions (m/z).Linearity was studied with 0.1–10μg/mL range in all corticosteroids. Good correlation coefficients (r2≥0.997) were found and the limits of quantification were 4.68–7.97ng/mL for each of the corticosteroids. At three different concentrations spanning the linear dynamic ranges, mean recoveries were 97.2–113.5%and precisions (RSD) for intra-day and inter-day analysis were less than 8.9%. Also, accuracy (Bias %) was less than 11.8%.The results showed that between 0.76–0.94μg/g levels of prednisolone were detected in four cosmetic products and triamcinolone acetonidewas detected with a concentration in the range of 11.5–272μg/g in nine samples. This fact reveals that some manufacturers have arbitrarily added these corticosteroids in their cosmetic products without indicating them on the label. Thus, these cosmetic products have to be monitored and if proven illegal preparations removed from the market.

The use of moxibustion scars in the identification of individuals in forensic medicine - Corrected Proof

2012-01-27

Abstract: The use of moxibustion today does not only take place in Eastern Asia, but recently also in Europe and the United States. Moxibustion scars are often seen in autopsy cases in Japan. However, no reports of the use of moxibustion in autopsy cases have been reported. This paper reports on an autopsy case in which moxibustion scars were used to extrapolate on the past symptoms of the deceased, date of therapy and the location of the moxibustion clinic which administered the moxibustion.

A study of quantitative comparisons of photographs and video images based on landmark derived feature vectors - Corrected Proof

Krista F. Kleinberg, J. Paul Siebert — 2012-01-27

Abstract: An abundunce of surveillance cameras highlights the necessity of identifying individuals recorded. Images captured are often unintelligible and are unable to provide irrefutable identifications by sight, and therefore a more systematic method for identification is required to address this problem. An existing database of video and photograhic images was examined, which had previously been used in a psychological research project; material consisted of 80 video (Sample 1) and 119 photograhic (Sample 2) images, though taken with different cameras. A set of 38 anthropometric landmarks were placed by hand capturing 59 ratios of inter-landmark distances to conduct within sample and between sample comparisons using normalised correlation calculations; mean absolute value between ratios, Euclidean distance and Cosine θ distance between ratios. The statistics of the two samples were examined to determine which calculation best ascertained if there were any detectable correlation differences between faces that fall under the same conditions. A comparison of each face in Sample 1 was then compared against the database of faces in Sample 2. We present pilot results showing that the Cosine θ distance equation using Z-normalised values achieved the largest separation between True Positive and True Negative faces. Having applied the Cosine θ distance equation we were then able to determine that if a match value returned is greater than 0.7, it is likely that the best match will be a True Positive allowing a decrease of database images to be verified by a human. However, a much larger sample of images requires to be tested to verify these outcomes.

Maximum flight velocity of blood drops in analysing blood traces - Corrected Proof

Beat P. Kneubuehl — 2012-01-25

Abstract: When analysing blood spatters, traces often occur which regarding the collision angle, cannot be allocated to any supposed centre of origin. Drops following highly curved (ballistic) trajectories usually form these types of traces. The reconstruction of such trajectories requires knowledge of the mass, the diameter (of which approximations are known) and the velocity of the blood drops. This article provides an upper range of the velocity in relation to the diameter of the blood drops based on physical laws. This is very helpful in analysing ballistic trajectories.

Experimental study of the expansion dynamic of 9mm Parabellum hollow point projectiles in ballistic gelatin - Corrected Proof

2012-01-24

Abstract: We study in this paper the expanding behaviour of hollow point 9mm Parabellum projectiles (Hornady XTP® and Speer Gold Dot®). We defined a deformation rate that takes into account both the diameter increase and the length reduction. We plotted the behaviour of this parameter versus impact velocity (we refer to this curve as the expanding law). This expanding law has been plotted for different gelatin weight ratios and different gelatin block lengths. We completed our experiments with a set of high speed movies in order to correlate the deceleration to the state of expansion and size of the temporary cavity. Our results pointed out that full expansion is reached shortly after the projectile fully penetrates the gelatin. This result shows that the key point to accurately simulate human body interaction with a hollow point projectile is to accurately simulate the interface (skin, skull, clothes thoracic walls). Simulating accurately organs is only an issue if a quantitative comparison between penetration depths is required, but not if we only focus on the state of expansion of the projectile. By varying the gelatin parameters, we discovered that the expanding law exhibits a velocity threshold below which no expansion occurs, followed by a rather linear curve. The parameters of that expanding law (velocity threshold and line slope) vary with the gelatin parameters, but our quantitative results demonstrate that these parameters are not extremely critical. Finally, our experiments demonstrate that the knowledge of the expansion law can be a useful tool to investigate a gunshot in a human body with a semi-jacketed projectile, giving an estimation of the impact velocity and thus the shooting distance.

Dental age estimation from the developmental stage of the third molars in western Chinese population - Corrected Proof

2012-01-24

Abstract: The purpose of this study is to provide reference data about estimating dental age from third molars of the western Chinese population for comparing with other populations and being applied to the age estimation of western Chinese juveniles and adolescents. A total of 2078 digital panoramic radiographs of 989 male and 1089 female Chinese subjects aged between 5 and 23 years were examined. The mineralization status of the third molars was assessed using the formation stages described by Demirjian et al. with two modifications. The results showed that the development of third molars in the western Chinese population was likely to begin at age 5 in both males and females. The third molars 28 and 48 showed significantly higher frequency in females than in males. The third molars 18 in the stage 1, 38 in the stages 1, A and G, and 48 in the stage H showed significantly older average age in females than in males. The Demirjian's stages C and D could be used as a reference stage to determine dichotomously whether a western Chinese is more likely to be under or above age 14 or 16, respectively. This study provided reference data for the age estimation of western Chinese juveniles and adolescents by the mineralization stages of the third molar. Apart from forensic age determination in living subjects, the presented reference data can also be used for age estimations of unidentified corpses and skeletons.

Screening and quantification of antipsychotic drugs in human brain tissue by liquid chromatography–tandem mass spectrometry: Application to postmortem diagnostics of forensic interest - Corrected Proof

2012-01-24

Abstract: A quantitative LC–MS/MS method has been developed for the simultaneous determination of 17 antipsychotic drugs in human postmortem brain tissue. Sample preparation was performed using Hybrid Solid Phase Extraction-Precipitation technology for the removal of endogenous protein and phospholipid interferences. The chromatographic separation was performed for 16min on a C8 column, which used a gradient elution of formate ammonium and acetonitrile, and a flow rate gradient. Triple quadrupole mass spectrometry was employed to generate tandem mass spectrometric (MS/MS) data of the target analytes to select the ion m/z signals. Quantitation of the analytes was performed by operating in the dynamic multiple reaction monitoring (dMRM) mode using an electrospray ionization interface. Calibration curves prepared in the spiked brain tissue were linear in the range 20–8000ng/g (r2>0.993) for all drugs (except olanzapine). Within- and between-day coefficients of variation were lower than 25% for all drugs at the LOQ. The LOQ in the matrix ranged between 2ng/g and 80ng/g. The method was successfully applied to the unequivocal identification and accurate quantification of antipsychotic drugs in human postmortem brain tissues: therefore, this method can be used in forensic investigations.

Informing the judgments of fingerprint analysts using quality metric and statistical assessment tools - Corrected Proof

Glenn Langenburg, Christophe Champod, Thibault Genessay — 2012-01-24

Abstract: The aim of this research was to evaluate how fingerprint analysts would incorporate information from newly developed tools into their decision making processes. Specifically, we assessed effects using the following: (1) a quality tool to aid in the assessment of the clarity of the friction ridge details, (2) a statistical tool to provide likelihood ratios representing the strength of the corresponding features between compared fingerprints, and (3) consensus information from a group of trained fingerprint experts. The measured variables for the effect on examiner performance were the accuracy and reproducibility of the conclusions against the ground truth (including the impact on error rates) and the analyst accuracy and variation for feature selection and comparison.The results showed that participants using the consensus information from other fingerprint experts demonstrated more consistency and accuracy in minutiae selection. They also demonstrated higher accuracy, sensitivity, and specificity in the decisions reported. The quality tool also affected minutiae selection (which, in turn, had limited influence on the reported decisions); the statistical tool did not appear to influence the reported decisions.

Anabolic androgenic steroids in police cases in Sweden 1999–2009 - Corrected Proof

Yvonne Lood, Arne Eklund, Mats Garle, Johan Ahlner — 2012-01-24

Abstract: Anabolic Androgenic Steroids (AAS) are considered drugs of abuse and are controlled substances in Sweden since 1999. Traditionally AAS have been used by elite athletes to enhance performance, but in recent years it has become an increasing problem outside elite sport among athletes, bodybuilders and criminals. Use of AAS is associated with psychiatric side effects such as aggression, depression and violent behavior. Supraphysiological doses and long term use can cause serious physical harm such as cardiovascular toxicity and even premature death. We investigated and evaluated the drug analytical findings in forensic cases from suspected perpetrators in cases from the police where a screening for AAS was requested to get information about the prevalence of AAS use and the occurrence of poly-drug abuse. The study was based on samples submitted from the police authorities to the Department of Forensic Toxicology in Sweden during the period 1999–2009. Urines were analyzed by methods based on GC–MS and LC–MS–MS. We also analyzed the prevalence of AAS use at the prison and probation services. A total number of 12,141 urine samples (6362 police cases and 5779 inmates) were analyzed and 33.5% of the cases from the police and 11.5% of the inmates were tested positive for AAS. The users of AAS were mainly in 99.2% men with a mean age of 26.2±6.2years whereas the women were 29.5±6.5years old. The most frequently used AAS was nandrolone followed by testosterone and methandienone. Other illicit and licit drugs were detected in 60% of the cases from the police, strongly indicating a frequent poly-drug abuse among users of AAS.

Separation and structural characterization of the synthetic cannabinoids JWH-412 and 1-[(5-fluoropentyl)-1H-indol-3yl]-(4-methylnaphthalen-1-yl)methanone using GC–MS, NMR analysis and a flash chromatography system - Corrected Proof

2012-01-20

Abstract: The ‘herbal highs’ market continues to boom. The added synthetic cannabinoids are often exchanged for another one with a high frequency to stay at least one step ahead of legal restrictions. While most of these substances were synthesized for pharmaceutical purposes and have been described in the scientific literature before, others originate from clandestine laboratories supplying this lucrative market. In this paper, the identification and structure elucidation of two synthetic cannabinoids is reported. The first compound, 1-[(5-fluoropentyl)-1H-indol-3yl]-(4-methylnaphthalen-1-yl)methanone, was found along with AM-2201 in a ‘herbal mixture’ obtained via the Internet. For isolation of the substance from the mixture, a newly developed flash chromatography method was used providing an inexpensive and fast way to gain pure reference substances from ‘Spice’ products for the timely development or enhancement of analytical methods in the forensic field. The second substance, 4-fluoronaphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-412) was seized by German authorities as microcrystalline powder, making it very likely that it will be found in ‘herbal mixtures’ soon.

A study of the evaporation of a solvent from a solution—Application to writing ink aging - Corrected Proof

Antonio A. Cantú — 2012-01-19

Abstract: When writing ink is placed on a substrate, a drying process begins. This process is dependent on the composition of the ink and of the substrate. Lociciro et al. provide an equation that describes the drying process based on models developed by earlier investigators. The work given here develops an equation for the drying process that is based on a different and rather simple model. This model considers the evaporation of a solution in an opened vertical container (e.g., a beaker) and consists of a volatile, non-hygroscopic solvent with a non-volatile solute dissolved in it. Three assumptions are made: (a) the rate of evaporation is proportional to the vapor pressure of the solution and to the solution's exposed surface area, (b) this solution vapor pressure is proportional to the solvent vapor pressure with the proportionality constant being the solvent mole fraction (Raoult's law), and (c) a small fraction of the solvent remains trapped in the solute after evaporation ceases. What results is a differential equation, which, when solved, gives the solvent weight W(t) as an implicit function. What emerges naturally from this treatment is the fact that the function W(t) can have a point of maximum acceleration. Prior to this point the drying process is fast and after this point, the drying process is slow. An approximation to W(t) is taken to be the sum of two exponential functions, one describing the fast drying region and the second describing the subsequent slow drying region. Upon including an additive constant, this approximation turns out to be similar to, but not the same as that provided by Lociciro et al. . However, their equivalence is shown and then tested using the two inks examined by Lociciro et al. (the drying of a Bic and a Staedtler blue ballpoint ink).The examples of (solvent+solute) systems (“inks”) given here consist of the solvent (2-phenoxyethanol) and a solute such as a dye (crystal violet) or a polymer resin such as synthetic resin SK or polyvinyl butyral. In the case of polymers, the Raoult's law assumption is replaced with one developed by the Flory–Huggins theory of solutions. Saturation and film formation, both of which slow the evaporation rate, are also discussed.

Application of Kvaal et al.’s age estimation method to panoramic radiographs from Turkish individuals - Corrected Proof

Hümeyra Özge Erbudak, Murat Özbek, Serdar Uysal, Erdem Karabulut — 2012-01-19

Abstract: Age estimation of living adult individuals can be accomplished with limited methods. Radiographic dental methods based on the pulpal narrowing with secondary dentin formation have been presented. In the present study, Kvaal et al.’s method, one of the radiographic dental age estimation methods, was applied to panoramic radiographs from Turkish individuals. The correlation between chronological and estimated ages was examined and the feasibility of length and width measurements of pulp cavity was evaluated for age estimation. The study population consisted of 123 patients with ages ranging from 14 to 57 years. The measurements of the length and width of six types of teeth on digitized panoramic radiographs were performed, and the ratios between tooth and pulp cavity measurements were calculated. Age was estimated using the linear regression models presented by Kvaal et al. and Paewinsky et al. High differences were observed between chronological and estimated ages. Measurement ratios showed no significant or weak correlation with age. The linear regression models were derived using variables that were significantly correlated with age. The determination coefficients of the models varied from 0.035 to 0.345. In conclusion, a difference of more than 12 years in the chronological and estimated ages derived using regression models in literature was found on panoramic radiographs in Turkish individuals. The length and width of the pulp cavity, measured according to the method of Kvaal et al. using panoramic radiographs, were insufficient to precisely estimate the age of Turkish individuals.

Real-time PCR detection and quantification of elephantid DNA: Species identification for highly processed samples associated with the ivory trade - Corrected Proof

Kristyne Michelle Wozney, Paul J. Wilson — 2012-01-18

Abstract: The ivory industry is the single most serious threat to global elephant populations. A highly sensitive, species-specific real-time PCR assay has been developed to detect and quantify African elephant (Loxodonta africana), Asian elephant (Elephas maximus) and Woolly Mammoth (Mammuthus primigenius) mitochondrial DNA from highly processed samples involved in the international ivory trade. This assay is especially useful for highly processed samples where there are no distinguishing morphological features to identify the species of origin. Using species-specific Taqman® probes targeting a region of the mitochondrial cytochrome b gene, we developed an assay that can be used to positively identify samples containing elephant or Woolly mammoth DNA faster and more cost-effectively than traditional sequencing methods. Furthermore, this assay provides a diagnostic result based on probe hybridization that eliminates ambiguities associated with traditional DNA sequence protocols involving low template DNA. The real-time method is highly sensitive, producing accurate and reproducible results in samples with as few as 100 copies of template DNA. This protocol can be applied to the enforcement of the Convention on the International Trade of Endangered Species (CITES), when positive identification of species from illegally traded products is required by conservation officers in wildlife forensic cases.

Mineralization of mandibular third molars can estimate chronological age—Brazilian indices - Corrected Proof

2012-01-18

Abstract: Forensic age estimation is an important element of anthropological research, as it produces one of the primary sources of data that researchers use to establish the identity of a person living or the identity of unknown bodily remains. The aim of this study was to determine if the chronology of third molar mineralization could be an accurate indicator of estimated age in a sample Brazilian population. If so, mineralization could determine the probability of an individual being 18years or older. The study evaluated 407 panoramic radiographs of males and females from the past 5years in order to assess the mineralization status of the mandibular third molars. The evaluation was carried out using an adaptation of Demirjian's system. The results indicated a strong correlation between chronological age and the mineralization of the mandibular third molars. The results indicated that modern Brazilian generation tends to demonstrate an earlier mandibular third molar mineralization than older Brazilian generation and people of other nationalities. Males reached developmental stages slightly earlier than females, but statistically significant differences between the sex were not found. The probability that an individual with third molar mineralization stage H had reached an age of 18years or older was 96.8–98.6% for males and females, respectively.

The PMMA epidemic in Norway: Comparison of fatal and non-fatal intoxications - Corrected Proof

2012-01-18

Abstract: During a 6 month period (July 2010–January 2011) we observed 12 fatal intoxications and 22 non-fatal cases related to the drug paramethoxymethamphetamine (PMMA) in Norway (4.8 mill inhabitants). This toxic designer drug, also known as “Death”, is occasionally found in street drugs offered as “ecstasy” or “amphetamine”. The present study aimed to evaluate the cause of death, and to compare the PMMA blood concentrations in fatal and non-fatal cases. Methods for identification and quantification of PMMA are presented. The median age of fatalities was 30 years (range 15–50) with 67% males; in non-fatal cases 27 years (20–47) with 86% males. In the 12 fatalities, the median PMMA blood concentration was 1.92mg/L (range 0.17–3.30), which is in the reported lethal range of 0.6–3.1mg/L in peripheral blood and 1.2–15.8mg/L in heart blood. In the 22 non-fatal cases, the median PMMA concentration was 0.07mg/L (range 0.01–0.65). Poly-drug use was frequent both in fatal and non-fatal cases. The PMA concentrations ranging from 0.00 to 0.26mg/L in both groups likely represented a PMMA metabolite. Three fatalities were attributed to PMMA only, six to PMMA and other psychostimulant drugs, and three to PMMA and CNS depressant drugs, with median PMMA concentrations of 3.05mg/L (range 1.58–3.30), 2.56 (1.52–3.23) and 0.52mg/L (0.17–1.24), respectively. Eight victims were found dead, while death was witnessed in four cases, with symptoms of acute respiratory distress, hyperthermia, cardiac arrest, convulsions, sudden collapse and/or multiple organ failure. In summary, all fatalities attributed to PMMA had high PMMA blood concentrations compared to non-fatal cases. Our sample size was too small to evaluate a possible impact of poly-drug use. A public warning is warranted against use and overdose with illegal “ecstasy” or “speed” drugs.

Detection of fingermarks by colloidal gold (MMD/SMD) – beyond the pH 3 limit - Corrected Proof

Andy Becue, Aurèle Scoundrianos, Sébastien Moret — 2012-01-10

Abstract: This work is part of a continuing goal to improve the multimetal deposition technique (MMD), as well as the single-metal deposition (SMD), to make them more robust, more user-friendly, and less labour-intensive. Indeed, two major limitations of the MMD/SMD were identified: (1) the synthesis of colloidal gold, which is quite labour-intensive, and (2) the sharp decrease in efficiency observed when the pH of the working solution is increased above pH 3. About the synthesis protocol, it has been simplified so that there is no more need to monitor the temperature during the synthesis. The efficiency has also been improved by adding aspartic acid, conjointly with sodium citrate, during the synthesis of colloidal gold. This extends the range of pH for which it is possible to detect fingermarks in the frame of the MMD/SMD. The operational range is now extended from 2 to 6.7, compared to 2–3 for the previous formulations. The increased robustness of the working solution may improve the ability of the technique to process substrates that tend to increase the pH of the solution after their immersion.

Identification process in mass graves from the Spanish Civil War II - Corrected Proof

Luis Ríos, Almudena García-Rubio, Berta Martínez, Andrea Alonso, Jorge Puente — 2012-01-09

Abstract: The identification process of a mass grave from the Spanish Civil War (1936–1939) is presented. The presumed location of the grave, as well as the presumed number and identities of the persons buried in the grave were obtained exclusively from witnesses’ and relatives’ testimonies. In agreement with the testimonies, the grave was located at the indicated location and five skeletons were exhumed. Also in agreement with the testimonies, the osteological and DNA study led investigators to propose the identification of two kin groups, a father and his son and a pair of brothers. But the genetic study did not support the identification of a fifth man presumed to have been buried in the grave. The differences and similarities between this case and another case reported earlier are discussed.

Raman spectroscopy and laser desorption mass spectrometry for minimal destructive forensic analysis of black and color inkjet printed documents - Corrected Proof

2012-01-09

Abstract: Inkjet ink analysis is the best way to discriminate between printed documents, or even though more difficult, to connect an inkjet printed document with a brand or model of printers. Raman spectroscopy and laser desorption mass spectrometry (LDMS) have been demonstrated as powerful tools for dyes and pigments analysis, which are ink components. The aim of this work is to evaluate the aforementioned techniques for inkjet inks analysis in terms of discriminating power, information quality, and nondestructive capability. So, we investigated 10 different inkjet ink cartridges (primary colors and black), 7 from the HP manufacturer and one each from Epson, Canon and Lexmark. This paper demonstrates the capabilities of three methods: Raman spectroscopy, LDMS and MALDI-MS. Raman spectroscopy, as it is preferable to try the nondestructive approach first, is successfully adapted to the analysis of color printed documents in most cases. For analysis of color inkjet inks by LDMS, we show that a MALDI matrix (9-aminoacridine, 9AA) is needed to desorb and to ionize dyes from most inkjet inks (except Epson inks). Therefore, a method was developed to apply the 9AA MALDI matrix directly onto the piece of paper while avoiding analyte spreading. The obtained mass spectra are very discriminating and lead to information about ink additives and paper compositions. Discrimination of black inkjet printed documents is more difficult because of the common use of carbon black as the principal pigment. We show for the first time the possibility to discriminate between two black-printed documents coming from different, as well as from the same, manufacturers. Mass spectra recorded from black inks in positive ion mode LDMS detect polyethylene glycol polymers which have characteristic mass distributions and end groups. Moreover, software has been developed for rapid and objective comparison of the low mass range of these positive mode LDMS spectra which have characteristic unknown peaks.

Fundamental studies of bloodstain formation and characteristics - Corrected Proof

Craig D. Adam — 2012-01-09

Abstract: A detailed understanding of blood droplet impact dynamics and stain formation is an essential prerequisite to the interpretation of both individual bloodstains and spatter patterns. The current literature on theoretical models for the spreading and splashing of liquid drops on surfaces relevant to the forensic context of bloodstain formation has been reviewed. These models have been evaluated for a paper substrate using experimental data obtained as function of droplet size, impact velocity and angle. It is shown that for perpendicular impact there are fairly simple mathematical models for the spreading diameter and the number of scallops or spines formed around the stain though these have quite limited ranges of validity in their basic form. In particular, predictions for the diameter are best for small droplets impacting at high velocity and the number of spines saturates for higher impact velocities. In the case of spreading, a modification to the energy conservation model is found to provide excellent agreement with experimental stain diameters across a wide range of impact velocities. For non-perpendicular impact, the width of stains is found to depend principally on the normal component of impact velocity and may be predicted by an appropriate modification to the expression for the perpendicular case. Limitations in the calculation of impact angle from the stain aspect ratio are identified and a theoretical basis for the prediction of spines around an elliptical stain is proposed. Some key issues for future research are identified which include a systematic, quantitative study of the effect of surface properties on bloodstain formation.

Comparison of quantitative PCR and culture-based methods for evaluating dispersal of Bacillus thuringiensis endospores at a bioterrorism hoax crime scene - Corrected Proof

Taryn Crighton, Rebecca Hoile, Nicholas V. Coleman — 2012-01-09

Abstract: Since the anthrax mail attacks of 2001, law enforcement agencies have processed thousands of suspicious mail incidents globally, many of which are hoax bioterrorism threats. Bio-insecticide preparations containing Bacillus thuringiensis (Bt) spores have been involved in several such threats in Australia, leading to the requirement for rapid and sensitive detection techniques for this organism, a close relative of Bacillus anthracis. Here we describe the development of a quantitative PCR (qPCR) method for the detection of Bt crystal toxin gene cry1, and evaluation of the method's effectiveness during a hoax bioterrorism event in 2009. When combined with moist wipe sampling, the cry1 qPCR was a rapid, reliable, and sensitive diagnostic tool for detecting and quantifying Bt contamination, and mapping endospore dispersal within a mail sorting facility. Results from the cry1 qPCR were validated by viable counts of the same samples on Bacillus-selective agar (PEMBA), which revealed a similar pattern of contamination. Extensive and persistent contamination of the facility was detected, both within the affected mailroom, and extending into office areas up to 30m distant from the source event, emphasising the need for improved containment procedures for suspicious mail items, both during and post-event. The cry1 qPCR enables detection of both viable and non-viable Bt spores and cells, which is important for historical crime scenes or scenes subjected to decontamination. This work provides a new rapid method to add to the forensics toolbox for crime scenes suspected to be contaminated with biological agents.

Quantitative determination of sibutramine in adulterated herbal slimming formulations by TLC-image analysis method - Corrected Proof

2012-01-09

Abstract: A simple thin layer chromatographic (TLC)-image analysis method was developed for rapid determination and quantitation of sibutramine hydrochloride (SH) adulterated in herbal slimming products. Chromatographic separation of SH was achieved on a silica gel 60 F254 TLC plate, using toluene-n-hexane-diethylamine (9:1:0.3, v/v/v) as the mobile phase and Dragendorff reagent as spot detection. Image analysis of the scanned TLC plate was performed to quantify the amount of SH. The polynomial regression data for the calibration plots showed good linear relationship in the concentration range of 1–6μg/spot. The limits of detection and quantitation were 190 and 634ng/spot, respectively. The method gave satisfactory specificity, precision, accuracy, robustness and was applied for determination of SH in herbal formulations. The contents of SH in adulterated samples determined by the TLC-image analysis and TLC-densitometry were also compared.

Evaluation of the catalytic decomposition of H2O2 through use of organo-metallic complexes – A potential link to the luminol presumptive blood test - Corrected Proof

2012-01-09

Abstract: Forensic scientists use several presumptive tests to detect latent blood stains at crime scenes; one of the most recognizable being the luminol reagent. Luminol, under basic conditions, reacts with an oxidizing species which, with the help of a transition metal catalyst facilitates a luminescent response. The typical oxidizing species used in the luminol reaction is hydrogen peroxide (H2O2). While the luminol reaction has been studied since its inception, the mechanistic pathway is still an area of great debate. Previous work suggests that the luminol reaction with latent blood stains possesses a correlation to the Fenton-Decomposition reaction mechanism, which decomposes H2O2 into the strongly oxidizing hydroxyl radical (*OH) species. This work seeks to understand the luminol reaction on a mechanistic level and to determine if a synergy exists between the chemiluminescence observed in the reaction and the production of the hydroxyl radical via Fenton-like processes. Results indicate that organo-metallic complexes produce hydroxyl radicals at different rates and different concentrations. These findings appear to be related to structural differences in the organo-metallic complex, which conform to the 18 electron rule or are one electron rich/deficient. Furthermore, the production of *OH is controlled by the chemical environment which governs complex stability at high pH conditions, reflective of the luminol process. Model hemoglobin systems reveal a strong correlation between the rate of *OH production via the Fenton-like pathway and maximum chemiluminescent intensity.

Fatal hypernatraemia after laparoscopic treatment of hydatid liver cyst: Medical and legal concerns of a rare complication - Corrected Proof

2012-01-04

Abstract: A case of fatal hypernatraemia after laparoscopic treatment of hydatid livers cysts is presented in order to highlight the risks associated with the implementation of newer techniques and the use of hypertonic saline as a scolecocidal agent in hydatid disease. Additionally this case raises some concerns on the importance of obtaining a patient's informed consent.

Estimating sex of the human skeleton based on metrics of the sternum - Corrected Proof

Rosanne Bongiovanni, M. Katherine Spradley — 2011-12-30

Abstract: Estimating the sex of skeletal remains is critical in creating the biological profile of an adult individual. Previous research has shown that analyzing the sternum may lead to an accurate estimation of sex based on studies performed on various populations around the globe, such as Indian, European, African, Canadian and North American. The motivation of the current study is to develop classification functions and sectioning points for use in forensic investigations in the United States. The majority of previously published methods are population specific, meaning the data would not prove useful in the United States .For this study, sternal measurements were collected from the William M. Bass Donated Skeletal Collection located at The University of Tennessee, Knoxville following the metric definitions provided by Schwartz and Bass . This collection consists of recent skeletal remains with known age at death, ancestry and sex. Material for the present study consisted of 410 human sterna: 285 male (256 American White/29 American Black) and 125 female (120 American White/5 American Black).Previous studies analyze the applicability of Hyrtl's law in the estimation of sex. This law follows that the mesosternum is greater than twice the length of the manubrium in males and in females the length of the manubrium is greater than half the length of the mesosternum. In this study, comparisons of the proportion of the length of the manubrium to the length of the mesosternum were performed to determine if Hrytl's law is applicable in an American population. Comparisons of these measurements between individuals identified as American Black and American White were analyzed to determine whether this method could be used on both population groups. Further, discriminant function analysis was used to estimate sex and provide a population specific classification function for use in the United States.The discriminate function analysis produced an overall cross-validation classification rate of 84.12% for sex estimation. The cross-validation classification rate for males and females was 80.00% and 88.24%, respectively.

Do century-specific equations provide better estimates of stature? A test of the 19–20th century boundary for the stature estimation feature in Fordisc 3.0 - Corrected Proof

John Albanese, Stephanie E. Osley, Andrew Tuck — 2011-12-29

Abstract: A sample (n=28) from the Terry Collection was selected to include only White males who were born and had their entire growth and development period before 1900 to assess the effects year of birth have on accuracy and precision when estimating stature. Using the computer application Fordisc 3.0, stature was estimated using the humerus, radius, femur, and tibia equations developed from White males born in the 19th Century and the 20th Century. The 19th Century White male equations did not consistently provide the most precise and accurate estimates of stature. The 20th Century equations provide results that were as good as or slightly better than the 19th Century equations for the humerus, radius and femur. The 20th Century equations provided notably better results for the tibia. There is a great deal of evidence that there are clear positive secular changes in most of North America in the last 100–125 years, but the division commonly advocated in a forensic context at the year 1900 has no positive effect on accuracy or precision when estimating stature.

Estimation of age at death from the pubic symphysis and the auricular surface of the ilium using a smoothing procedure - Corrected Proof

Rui Martins, Paulo Eduardo Oliveira, Aurore Schmitt — 2011-12-29

Abstract: We discuss here the estimation of age at death from two indicators (pubic symphysis and the sacro-pelvic surface of the ilium) based on four different osteological series from Portugal, Great-Britain, South Africa or USA (European origin). These samples and the scoring system of the two indicators were used by Schmitt et al. (2002) , applying the methodology proposed by Lucy et al. (1996) . In the present work, the same data was processed using a modification of the empirical method proposed by Lucy et al. (2002) . The various probability distributions are estimated from training data by using kernel density procedures and Jackknife methodology. Bayes's theorem is then used to produce the posterior distribution from which point and interval estimates may be made. This statistical approach reduces the bias of the estimates to less than 70% of what was obtained by the initial method. This reduction going up to 52% if knowledge of sex of the individual is available, and produces an age for all the individuals that improves age at death assessment.

Spatial patterning of vulture scavenged human remains - Corrected Proof

M. Katherine Spradley, Michelle D. Hamilton, Alberto Giordano — 2011-12-28

Abstract: This article presents the results of a pilot study on the effects of vulture modification to human remains. A donated body from the Willed Body Donation Program was placed at the Forensic Anthropology Research Facility (FARF), an outdoor human decomposition laboratory located at Texas State University-San Marcos. The effects of vulture scavenging on the timing and sequence, and the rate of skeletonization, disarticulation, and dispersal were observed via a motion sensing camera and direct observation. Using GIS (Geographic Information Systems) and GPS (Global Positioning System) technologies and spatial analytical methods, the transport of skeletal elements was mapped in order to analyze dispersal and terrain-influenced patterns of active vulture scavenging. Results showed that the initial scavenging took place 37 days after placement at FARF. This delay in scavenging differs from previous research . After the initial appearance of the vultures, the body was reduced from a fully-fleshed individual to a skeleton within only 5h. This underscores the potential for errors in postmortem interval estimations made at vulture scavenged scenes. Additionally, spatial analysis showed that skeletal elements were dispersed by vultures to lower elevations, and that the disarticulation and dispersal of the skeletal elements occurs early in the scavenging sequence.

First practical applications of eye temperature measurements for estimation of the time of death in casework. Report of three cases - Corrected Proof

Michał Kaliszan — 2011-12-26

Abstract: This paper presents first three successive cases of death where at scene measurements of the internal eyeball temperature soon after death allowed to precisely estimate the times of deaths based on this data (33.1°C, 32.2°C and 29.5°C, respectively), which were later confirmed during the police investigation. Simultaneously the rectal temperatures in all three cases were measured and appeared to be between 36.3 and 36.8°C—reflecting a living individual's body temperature. Thanks to a significantly faster postmortem decrease of the eye temperature and a residual or nonexistent plateau effect affecting the eye, using the formula developed in previous comprehensive studies in pigs, the time of death in real cases could be estimated with good precision: 1h 33min, 2h 24min and 3h 17min since death respectively. The actual TOD established during investigation appeared to be: between 1h 30min and 1h 50min in case 1; 1h 55min in case 2 and between 2h 55min and 3h 05min in case 3. Such precise estimation, mainly due to the plateau effect lasting up to a few hours, could not have been done based only on rectal temperature, commonly measured in forensic practice.

Analyses of sexual dimorphism of reconstructed pelvic computed tomography images of contemporary Japanese using curvature of the greater sciatic notch, pubic arch and greater pelvis - Corrected Proof

2011-12-26

Abstract: Three-dimensional pelvic images were reconstructed from multi-slice CT data of contemporary Japanese (males: 124; females: 104, 25–92 years old), and curvature analysis to examine sexual dimorphism was carried out in the great sciatic notch (GSN), the pubic arch and the greater pelvis in the images. Reconstructed pelvic CT images were visualized fairly well and anatomical landmarks were easily recognizable. When calculating the radii (curvature radii) of the best-fit circles for the spline curve lines set along the edges of the GSNs and of the pubic arches, sexes from these regions were correctly identified in 89.1% (males: 93.8%; females: 83.7%) and 94.7% (males: 97.3%; females: 91.8%) of cases, respectively, by setting an appropriate cut-off value. Furthermore, sexing was possible even in deeper regions of the GSN which are relatively resistant to postmortem damage. Curvature radii of the best-fit spheres of greater pelves showed no significant difference between sexes. However, curvature of the best-fit sphere for the left iliac fossa was significantly larger than that of the right one (p<10−24) in males, and the ratios were >1.0 in 88% of all male specimens analyzed. Meanwhile, no significant difference was observed among female samples. Although some left-sided dominancy has been reported in 2-dimensional measurements of the human pelvis, this 3-dimensional laterality in males was much more significant, and is a potential index of sex difference.

Diploe thickness and cranial dimensions in males and females in mid-Anatolian population: An MRI study - Corrected Proof

2011-12-26

Abstract: Background: The objective of this study is the classification of the thickness of diploe and dimensions of cranium at different points of cranium in men and women according to age groups.Material and method: In the radiology archive, measurements were made at different points in magnetic resonance (MR) images of 305 (188 females and 117 males) patients, the average ages of whom were 40.98±20.44 (age range: 4–90) and who had no disorder of the bones. To determine diploe thickness, midfrontal, back and front bregma, lambda, opisthocranion and euryon points were used in the measurement. As for the determination of cranial volume, distances between glabella–opisthocranion, basion–vertex, basion–opisthion, euryon–euryon, nasion–basion, nasion–bregma, bregma–lambda and lambda–opisthocranion were measured. The data were loaded to Statistical Package for the Social Sciences (SPSS) 16.0 program. T-test, Mann–Whitney U, Pearson correlation coefficient and Kruskal–Wallis variance analysis were used in the statistical assessment. Results with a p value smaller than 0.05 were accepted as significant.Results: There was a statistically significant positive correlation between age and diploe thickness in all measurement points. The diploe thickness was also increased with age (p<0.05). In all points, average diploe thickness was higher in age 61 and over than the other groups (p<0.001). At the same time, diploe thickness in parietal bones was lower than frontal and occipital bones in both sexes.According to craniometric results cranium in males was bigger (p<0.001).While the distance between glabella–opisthocranion increased in both sexes aged 61 and over, basion–vertex height decreased in women in the same group (p<0.05).Interestingly, there was no meaningful statistical difference among age groups in terms of maximum cranial width (p>0.05).Foramen magnum length decreased related to age in both men and women (p<0.001).Conclusion: These results related to diploe thickness and cranium dimension may be leading in the determination of sex and age; surgical interventions to the cranium and bone graft choice may increase the reliability of the operation.

Nature, frequency and duration of genital lesions after consensual sexual intercourse—Implications for legal proceedings - Corrected Proof

Birgitte Schmidt Astrup, Pernille Ravn, Jens Lauritsen, Jørgen Lange Thomsen — 2011-12-22

Abstract: Objective: The purpose of this study was to make a normative description of the nature and duration of genital lesions sustained during consensual sexual intercourse, using the three most commonly used techniques; visualisation using the naked eye, colposcopy and toluidine blue dye followed by colposcopy.Methods: Ninety eight women were examined within 48h of consensual sexual intercourse. Fifty of the women were examined twice again within the following 7 days of sexual abstinence after the first examination.Results: The participants had a median age of 22.4 years and 88% were nulliparous. Lesions were frequent; 34% seen with the naked eye, 49% seen with colposcopy and 52% seen with toluidine blue dye and subsequent colposcopy. The lesions lasted for several days; the median survival times for lacerations were 24, 40 and 80h, respectively.Conclusions: The legal implications of these findings are that genital lesions by themselves do not corroborate a rape complaint. Genital lesions may, however, corroborate specific details of a case and should be documented as carefully as any other lesion in rape complaints.

Managing commingled remains from mass graves: Considerations, implications and recommendations from a human rights case in Chile - Corrected Proof

Claudia Garrido Varas, Marisol Intriago Leiva — 2011-12-22

Abstract: This paper focuses on a little discussed part of Chilean history and the efforts to resolve Human Rights cases from the period 1973 to 1990.A case file is presented to illustrate the different stages, problems and solutions found in one particularly challenging case studied by the Special Unit of Identification of Detained and Missing (UEIDDDD) of the Human Rights Program of the Forensic Service, Chile, during the period 2006–2009. A major complication found in this example was the fact that the remains studied were commingled, and in addition, were mixed with remains that were not of medico-legal interest – deposited prior to and after the case in question, but within the same common grave.Multiple burials and the consequences of commingled skeletal human remains are reviewed, paying special attention to the roles that different agents related to these types of cases play, how they influence the decision making process and the outcomes that can be expected in commingled cases due to the complexities and challenges they present.Effective communication between prosecutors and forensic practitioners is vital to optimize the resources. Equally important is the relationship that is built between the practitioners and the victims’ families regarding communication, information and expectations that both parties may have.

Adult Fanniidae associated to pig carcasses during the winter season in a semiarid environment: Initial examination of their potential as complementary PMI indicators - Corrected Proof

Fernando H. Aballay, M. Cecilia Domínguez, Florencia Fernández Campón — 2011-12-21

Abstract: Besides the dominant necrophagous dipteran of the families Sarcophagidae and Calliphoridae usually used for post mortem interval (PMI) estimations, species of other families such as Fanniidae have frequently been reported in forensic studies. Though less abundant, these species are prevalent in decomposing carcasses with most reports being anecdotal. In this study we identified adults of the fly family Fanniidae associated to pig carcasses located under different local environmental conditions (sun and shade) in a semiarid area at Mendoza, Argentina during the winter season. We examined the potential of species of this family as indicators of PMI by measuring abundance, time of occurrence and residency time at the carcasses. We identified six species of Fanniidae: Euryomma peregrinum Meigen, Fannia albitarsis Stein, Fannia femoralis Stein, Fannia fusconotata Rondani, Fannia heydenii Wiedemann and Fannia sanihue Domínguez and Aballay. Overall, fly abundance was higher at the sunlit than at the shaded carcass. The most abundant species at the sun was F. fusconotata while at the shaded carcass F. femoralis was the most abundant species. Based on their residency time, however, species with higher potential as PMI indicators seem to be F. heydenii and F. sanihue as their residency time at the carcass was restricted to a short period of the decomposition process. Other species were present throughout most of the decomposition process or in such a low abundance (E. peregrinum) that they were not useful as indicators. These preliminary results indicate that adults of some species of Fanniidae could act as a good complementary indicator species during the winter season. In particular, F. heydenii and F. sanihue should be the focus of further studies which should also expand to other seasons.

The effect of sodium fluoride preservative and storage temperature on the stability of cocaine in horse blood, sheep vitreous and deer muscle - Corrected Proof

2011-12-20

Abstract: The in vitro stability of cocaine in horse blood, sheep vitreous humour (VH) and homogenised deer muscle is described. The stability of cocaine in horse blood was of interest because many toxicology laboratories utilise horse blood for the preparation of calibration and check standards and the latter are typically stored during routine use. The storage stability of cocaine in human VH and muscle has not been previously reported. In the absence of blank human VH and muscle, cocaine stability under varying conditions was demonstrated in animal tissues. Blood and VH were stored with and without addition of NaF at room temperature (RT), 4°C and −18°C for 84 days. Muscle homogenates were prepared in water, water/2% NaF, and phosphate buffer (pH 6.0)/2% NaF, and stored for 31 days at RT, 4°C and −18°C. Cocaine stability in human muscle obtained from cocaine positive forensic cases was assessed following storage at −18°C for 13 months. Cocaine and benzoylecgonine (BZE) were extracted using SPE and quantified by GC-MS/MS. Cocaine was stable for 7 days in refrigerated (4°C) horse blood fortified with 1 and 2% NaF. In the absence of NaF, cocaine was not detectable by day 7 in blood stored at RT and 4°C and had declined by 81% following storage at −18°C. At 4°C the rate of cocaine degradation in blood preserved with 2% NaF was significantly slower than with 1% NaF. The stability of cocaine in horse blood appeared to be less than that reported for human blood, probably attributable to the presence of carboxylesterase in horse plasma. Cocaine stored in VH at −18°C was essentially stable for the study period whereas at 4°C concentrations decreased by >50% in preserved and unpreserved VH stored for longer than 14 days. Fluoride did not significantly affect cocaine stability in VH. The stability of cocaine in muscle tissue homogenates significantly exceeded that in blood and VH at every temperature. In preserved and unpreserved samples stored at 4°C and below, cocaine loss did not exceed 2%. The increased stability of cocaine in muscle was attributed to the low initial pH of post-mortem muscle. In tissue from one human case stored for 13 months at −18°C the muscle cocaine concentration declined by only 15% (range: 5–22%). These findings promote the use of human muscle as a toxicological specimen in which cocaine may be detected for longer compared with blood or VH.

The effect of sodium fluoride preservative and storage temperature on the stability of 6-acetylmorphine in horse blood, sheep vitreous and deer muscle - Corrected Proof

Kelly A. Rees, Nicola S. Jones, Poppy A. McLaughlin, M. David Osselton — 2011-12-20

Abstract: This study examined the in vitro stability of 6-acetylmorphine (6AM) in horse blood, sheep vitreous humour (VH) and homogenised deer muscle stored under different storage conditions. The stability of 6AM in horse blood is of interest because many toxicological laboratories utilise this matrix for the preparation of blood calibration and check standards and the latter are typically stored during routine use. Data on the storage stability of 6AM in human VH is extremely limited and no data has been reported in muscle. In the absence of human samples, 6AM stability was demonstrated in sheep vitreous and deer muscle. Blood and VH were stored with and without NaF at room temperature (RT), 4 and −18°C for 84 days. Muscle tissue homogenates were prepared in water with and without NaF and also in phosphate buffer (pH 6.0) containing NaF. Homogenates were stored for 31 days at RT, 4 and −18°C. Morphine and 6AM were extracted using SPE and quantified by GC–ion trap-MS/MS. In the absence of NaF, 6AM could not be detected after 7 and 14 days in blood stored at RT and 4°C, respectively. Although at −18°C 6AM was stable for 7 days (12% loss), only 54% was detected by day 84. The addition of NaF to horse blood increased 6AM stability substantially at every temperature. Further, the rate of degradation was found to be significantly slower in blood preserved with 2% NaF compared with 1% NaF (p=.05). 6AM was stable for the study period in preserved blood (1 and 2% NaF) stored at −18°C. For laboratories utilising horse blood in the preparation of standards, preservation with 1% NaF (minimum) and storage at −18°C is recommended. The addition of NaF to VH was essential for 6AM stability. Irrespective of temperature substantial losses (≥42%) were observed in unpreserved sheep VH by day 7. In preserved VH the concentration declined by only 22% on day 7 following storage at RT and no loss observed in VH stored at 4 and −18°C at the same time. In muscle, 6AM was stable for 7 days in preserved samples stored at RT and in all samples stored at 4°C and below. The addition of NaF increased the stability of 6AM substantially in muscle. The increased stability of 6AM in VH and muscle preserved with fluoride was attributed to inhibition of bacterial action and the subsequent reduction in the rate of putrefaction of these tissues.

Comments on “The database search problem” with respect to a recent publication in Forensic Science International - Corrected Proof

Anders Nordgaard, Karin Hedberg, Christina Widén, Ricky Ansell — 2011-12-20

In a recent paper in Forensic Science International the so-called “database search problem” or “database debate” is addressed, and the existing arguments within this debate are illustrated with the use of Bayesian networks. The database search problem relates to a series of articles and letters published during the period 1996–2007, of which the majority appeared in Biometrics. This debate seemed to have been closed upon the last article of the series, but most surprisingly it has been reopened. In a recent paper by Schneider et al. the authors present guidelines for handling the case where a DNA profile obtained from a crime scene matches the DNA profile of an individual in a database. These guidelines stress the use of the “np-rule”, which in simplified terms means that the likelihood ratio used as evidence value should be computed as 1/np, where n is the number of persons in the database and p is the random match probability, as opposed to 1/p, that is the corresponding value when the suspect has been identified by other reasons (the case of probable cause). For sake of simplicity, we from now on refer to these two types of cases as “a database hit case” and “a probable cause case” respectively.

Concentrations of trace element in human dentin by sex and age - Corrected Proof

Akiko Kumagai, Yuji Fujita, Shigeatsu Endo, Kazuyoshi Itai — 2011-12-16

Abstract: Teeth are recently drawing attention for their potential as biological modeling investigation samples due to their ability to be collected and their slow substance metabolism. There is no active metabolism of elements after the completion of dentin. Dentin is surrounded by enamel and cementum, and is not affected by the oral environment. Therefore, the amount of trace elements in dentin may change with age, and this is considered to be a reliable biological load index. The objectives in this study are to demonstrate concentrations of elements in the dentin of healthy Japanese subjects by sex and age, and to reveal the relationship between element levels and age. 121 healthy teeth samples were extracted due to periodontal disease or orthodontic treatment. Each tooth was sliced from the crown to the root apex into 0.5–1mm thickness, then enamel, cementum, and the pulp were removed; the dentins were used as samples. The concentration of 10 trace elements (B, Mn, Co, Cu, Zn, Rb, Sr, Mo, Cd, and Pb) in the dentin was measured using inductively coupled plasma mass spectrometer (ICP-MS). The differences Co and Pb in the dentin between men and women were significant (p<0.01). Significant positive correlation was observed between B, Co, Cu, Zn, Sr and Pb concentrations in the dentin and age (p<0.001). The results of the present study suggest that human dentin is an appropriate substance for relativity with sex and age at further future research.

Molecular autopsy in young sudden cardiac death victims with suspected cardiomyopathy - Corrected Proof

2011-12-16

Abstract: The aim of this investigation was to identify and characterise pathogenic mutations in a sudden cardiac death (SCD) cohort suspected of cardiomyopathy in persons aged 0–40 years.The study material for the genetic screening of cardiomyopathies consisted of 41 cases and was selected from the case database at the Institute of Forensic Medicine. Mutational screening by DNA sequencing was performed to detect mutations in DNA samples from deceased persons suspected of suffering from hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), and arrhythmogenic right ventricle cardiomyopathy (ARVC).A total of 9 of the examined 41 cases had a rare sequence variant in the MYBPC3, MYH7, LMNA, PKP2 or TMEM43 genes, of which 4 cases (9.8%) were presumed to be pathogenic mutations. The presumed pathogenic mutations were distributed with one case of suspected HCM and DCM (MYH7; p.R442H), one case of suspected DCM (LMNA; p.R471H), and two cases of suspected ARVC (PKP2; p.R79X and LMNA; p.R644C).The presented data adds important information on the genetic elements of SCD in the young, and calls for expert pathological evaluation and molecular autopsy in the post-mortem examination of SCD victims with structural anomalies of the heart.