Application of hypervariable genetic markers to forensic identification of ‘wild’ from hatchery-raised red drum, Sciaenops ocellatus

Abstract 

Forensic identification of ‘wild’ versus hatchery-produced (cultured) red drum (Sciaenops ocellatus), an economically important marine fish in the southern United States, was assessed using hypervariable nuclear-encoded microsatellites and sequences of mitochondrial DNA. Both genotype exclusion and likelihood-ratio tests successfully identified ‘wild’ and ‘cultured’ individuals within requisite error bounds and within the context of complete parental sampling. Of the two, genotype exclusion was more effective, producing satisfactory results with fewer microsatellites and larger allowable error rates. Assignment tests proved ineffective, most likely because of the low level of genetic divergence between the sampled populations. An optimal, minimum set of ten markers that will reduce potential genotyping costs is identified. Results of the study should allay concerns regarding identification of ‘wild’-caught fish sold illegally.

Keywords: Microsatellites, Forensic genetics, ‘Wild’ versus hatchery-raised fish