Forensic Science International
Volume 161, Issue 1 , Pages 1-7, 10 August 2006

26-Locus Y-STR typing in a Bhutanese population sample

  • Emma J. Parkin

      Affiliations

    • Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK
    • ,
  • Thirsa Kraayenbrink

      Affiliations

    • MGC-Department of Human and Clinical Genetics, Leiden University Medical Centre, The Netherlands
    • ,
  • George L. van Driem

      Affiliations

    • Himalayan Languages Project, Leiden University, The Netherlands
    • ,
  • Karma Tshering of Gaselô

      Affiliations

    • MGC-Department of Human and Clinical Genetics, Leiden University Medical Centre, The Netherlands
    • ,
  • Peter de Knijff

      Affiliations

    • MGC-Department of Human and Clinical Genetics, Leiden University Medical Centre, The Netherlands
    • ,
  • Mark A. Jobling

      Affiliations

    • Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 116 252 3427; fax: +44 116 252 3378.

Received 10 August 2005; received in revised form 9 October 2005; accepted 9 October 2005. published online 17 November 2005.

Abstract 

26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10–24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).

Keywords: Y chromosome, STRs, Microsatellites, Multiplex, Bhutan

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PII: S0379-0738(05)00582-7

doi:10.1016/j.forsciint.2005.10.008

Forensic Science International
Volume 161, Issue 1 , Pages 1-7, 10 August 2006

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