Forensic Science International
Volume 159, Issue 2 , Pages 168-174, 2 June 2006

Determination of tetramethylenedisulfotetramine in human urine with gas chromatograph-flame thermionic detection coupling with direct immersed solid-phase micro-extraction

  • Dong Zeng

      Affiliations

    • Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China
    • ,
  • Bo Chen

      Affiliations

    • Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China
    • Corresponding Author InformationCorresponding author. Tel.: +86 731 8865515; fax: +86 731 8865515.
    • ,
  • Shouzhuo Yao

      Affiliations

    • Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China
    • ,
  • Jianying Ying

      Affiliations

    • Hunan Public Security Department, Changsha 410001, China

Received 11 December 2004; received in revised form 22 July 2005; accepted 23 July 2005. published online 08 September 2005.

Abstract 

An analytical method for determination of tetramethylenedisulfotetramine (tetramine) in human urine by gas chromatography-flame thermionic detection (GC-FTD) coupling with a direct immersed solid-phase micro-extraction (DI-SPME) was developed. The enrichment effects of three fiber coatings of SPME for tetramine were compared. Results showed that the enrichment effect of polar 85μm polyacrylate (PA) coating was better than that of apolar 100 and 7μm polydimethylsiloxane (PDMS) coatings. Other experimental parameters, such as ionic strength, volume, temperature of sample solution and time for extraction, time and temperature for desorption, were also optimized. The correlation coefficient of the calibration curve was 0.9998 in the range of 0.082–41.0ng/mL for tetramine. The limit of quantitation of tetramine in urine was 0.082ng/mL. In this method, the sample pretreatment is simple and convenient. As a monitoring means, it has been successfully applied to detection of tetramine toxicosis in criminal cases, as well as clinical therapy of poisoned sufferer.

Keywords: Tetramethylenedisulfotetramine, Gas chromatography, Solid-phase micro-extraction, Urine

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PII: S0379-0738(05)00417-2

doi:10.1016/j.forsciint.2005.07.013

Forensic Science International
Volume 159, Issue 2 , Pages 168-174, 2 June 2006

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