Investigation of the accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) in rat kidneys

Abstract 

The accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) and its metabolite 2,4-dichlorophenol (2,4-DCP) in the kidneys of rats was investigated. Male and female Sprague–Dawley rats were given 2,4-D in drinking water and food for 30 days. Group A (control group) was fed a normal diet, Group B was fed 50ppm 2,4-D in 15g food, Group C received 100ppm 2,4-D in 15g food, Group D received 25ppm 2,4-D in 15ml drinking water and Group E was given 50ppm 2,4-D in 15ml of drinking water. Levels of 2,4-D and 2,4-DCP in kidneys were determined using high performance liquid chromatography (HPLC). It was observed that at low doses of 2,4-D, the metabolite, 2,4-DCP found in the kidneys.

Abbreviations: 2,4-D, 2,4-dichlorophenoxyacetic acid, 2,4-DCP, 2,4-dichlorophenol

Keywords: 2,4-Dichlorophenoxyacetic acid (2,4-D), 2,4-Dichlorophenol (2,4-DCP), HPLC, Kidney accumulation, Rat