A method for screening for various sedative-hypnotics in serum by liquid chromatography/single quadrupole mass spectrometry

Abstract 

A screening method for the detection of sedative-hypnotics in serum is described. The target drugs, which include practically all the sedative-hypnotics distributed in Japan, consisted of 5 barbiturates, 30 benzodiazepine-related drugs and 11 other sedative-hypnotics (i.e., apronalide, bromisovalum, chloral hydrate, triclofos, chlorpromazine, promethazine, diphenhydramine, hydroxyzine, zopiclone, zolpidem and tandospirone). Thirty-nine analytes, selected in terms of the pharmacokinetics of the target drugs, in human serum were screened using a combination of mixed-mode solid-phase extraction and liquid chromatography/electrospray-ionization single-quadrupole mass spectrometry. The detection limits (non-basic analytes, 1–50ng/ml; basic analytes, 0.1–5ng/ml) were sufficient to permit the screening of a single therapeutic administration of a target drug.

Keywords: Drug screening, Benzodiazepines, Serum, Solid-phase extraction, Liquid chromatography/mass spectrometry (LC/MS)