Forensic Science International
Volume 118, Issue 2 , Pages 163-168, 15 May 2001

The use of the LightCycler for the detection of Y chromosome SNPs

Institute of Legal Medicine, University of Santiago de Compostela, E-15705 Santiago de Compostela, Galicia, Spain

Received 25 October 2000; accepted 7 November 2000.

Abstract 

A novel methodology based on PCR monitoring on-line with fluorescent formats using the LightCycler for Y chromosome SNP typing is proposed. The main advantages of the system are the time necessary for the analysis (which is around 20min), the robustness and the accuracy of the method and especially its sensitivity, which permits the detection of the male component in male–female mixtures up to 1:300 for some of the SNPs.

Singleplexes of four different SNPs (M9, sY81, SRY-1532 and SRY-2627) as well as two duplexes (M9 and sY81 on the one hand and SRY-1532 and SRY-2627 on the other) were efficiently implemented. A simultaneous amplification and analysis of the four SNPs is also possible. It seems difficult with the current methodology to implement more than a quadruplex.

Keywords:  SNPs, Y chromosome, LightCycler, Mix stains

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PII: S0379-0738(01)00386-3

doi:10.1016/S0379-0738(01)00386-3

Forensic Science International
Volume 118, Issue 2 , Pages 163-168, 15 May 2001

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