Forensic Science International
Volume 118, Issue 2 , Pages 122-125, 15 May 2001

Application of a Y-STR-pentaplex PCR (DYS19, DYS389I and II, DYS390 and DYS393) to sexual assault cases

National Institute for Criminalistics and Criminology, Ch. De Vilvorde 98-100, B-1120 Brussels, Belgium

Received 2 November 2000; accepted 9 November 2000.

Abstract 

A survey of 89 random rape cases including 166 semen traces, according to the prostate-specific-antigen test, showed the need for a more efficient method of DNA-profiling. No male autosomal STR-profile could be detected, after differential lysis, in 35% of the 84 samples usually containing high amounts of the victim’s cells, i.e. vaginal/anal washings and knickers. A Y-STR-pentaplex was applied to the stored sperm fraction of 50 unsolved semen traces, with success in 48% of them. The chance of success depends of the amount of total DNA available and of the quality of the extracted DNA.

Keywords:  Y-STR-pentaplex, DNA-profiling, PCR

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PII: S0379-0738(00)00481-3

doi:10.1016/S0379-0738(00)00481-3

Forensic Science International
Volume 118, Issue 2 , Pages 122-125, 15 May 2001

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